Description
Scientist I: CLD Small Scale Bioreactor SME
An exciting opportunity is available for a Cell Line Development Scientist to join a growing start-up. With state-of-the-art facilities and a robust pipeline of upcoming projects, this role offers the chance to contribute to innovative research and development.
Responsibilities:
The purpose of this role is to develop and execute small scale (Ambr15 & 24/96 deep well) fed-batch methods to screen and validate cell lines as part an innovative multidisciplinary team that will develop the next generation of mammalian expression systems & cells lines to enable highly productive pharmaceutical bioprocesses and, ultimately, more cost effective Immunotherapeutics.
- Author SOPs, training documents & COSHH forms as key deliverables
- Train team members to a high degree of competence to ensure data integrity
- Document research findings clearly in a lab book
- Communicate regularly in group meetings and prepare and deliver presentations to project team and internal stakeholders
- Along with team members, provide routine laboratory maintenance, including participating in equipment upkeep, cleaning rotas and laboratory consumables stock management
- Shared responsibility for out-of-hours cell culture maintenance.
- Ensure that laboratory health, safety and quality procedures are followed
- Support the preparation of laboratory risk assessments and maintenance of health and safety protocols
- Maintain the confidentiality of the project and protocols
- Keep abreast of current technologies and relevant scientific literature to help identify opportunity for improvements and reduction of costs
Minimum Required Qualifications
- BSc/MSc in Biochemistry, Molecular Biology or related field
- BSc/MSc with 5+ years of industry experience or PhD with 2+ years’ experience
Essential Experience:
- Extensive experience of working with small scale bioreactor systems including Ambr15
- Experience of developing small scall mammalian cell culture fed batch models systems using deep well microplates
- Experience of screening clonal cell lines for optimal performance in batch, fed-batch and perfusion bioreactor systems
- Experience of optimising fed-batch methods for antibody bioproduction
- Experience of working with bioprocess relevant cell lines including CHO and HEK293
- Experience of performing analytical methods to characterise cell lines for high antibody titre, qP and CQA such as HPLC/UPLC & LC-MS
- Application of Design of Experiments (DOE) to rationalise experiment design
- Skilled in experimental design and troubleshooting
- Experience of writing SOPs, training documents and risk assessment
- Experience of training other team members to a high level of competency
- Familiarity of mammalian cell line development including generation of GM/GE cell lines, single cell cloning and CRISPR / ORF library screens
- Evidence of executing a range of molecular and cell biology assays to a high standard including FACS, qRT-PCR, Western Blotting & ELISA
- Ability to work independently and as part of team
- Experience of planning experiments with a given timeframe
- Good numeracy, computer and communication skills
Desirable Experience:
- Experience with automation and high-throughput technologies applied to mammalian cell line generation eg. Beacon, 96 deep well plate model systems & Ambr15
- Familiarity of working with large datasets including transcriptomic / proteomic datasets
- Experience of mammalian cell culture and related techniques including passaging, banking, transfection, nucleofection & genotyping
- Experience of working safely with 3rd generation lentiviral plasmid systems within a CAT2.0 environment
Skills and personality:
- Innovative mindset
- Advanced problem-solving skill
- Enthusiasm and initiative
- Driven to deliver
- Reliable and responsible
- Able to multitask
- Good time management and organisation skills
- Excellent communication skills both written and spoken
- Happy in a growing and multi-disciplinary team
